Serous Ovarian Cancer (SOC) Assignment
Implications Of Falls On Adults’ Health
April 15, 2021Cancer Targeted Therapy Assignment
April 15, 2021Serous Ovarian Cancer (SOC) Assignment
Serous Ovarian Cancer (SOC) Assignment
Here we sought to determine the relationship between STAT3 activity and Galectin-3 (Gal-3) and to investigate the cytotoxic effect of PectaSol-C Modified Citrus Pectin (Pect-MCP) as a specific competitive inhibitor of Galectin-3 (Gal-3) in combination with paclitaxel (PTX) to kill ovarian cancer cell multicellular tumor spheroid (MCTS). SKOV-3 cells in 2D and 3D culture were treated with exogenous Gal-3 for assessment of STAT3 activity. Two-way ANOVA main effect and IC50 of each drug Paclitaxel (PTX) and Pect-MCP or in combination were obtained from MTT assay results.Serous Ovarian Cancer (SOC) Assignment
The phosphorylated STAT3 levels, migration, invasion, integrin mRNA and p-AKTser473 levels were assessed in the absence or presence of each drug alone or in combination. Gal-3 expression levels were assessed in human serous ovarian cancer (SOC) specimens and its correlation with different integrin mRNA levels was further assessed. Gal-3 triggered STAT3 phosphorylation and its expression was significantly increased in MCTS compared to monolayer SKOV-3 cells. Moreover, Pect-MCP synergized with PTX to kill SKOV3 MCTS and abrogated STAT3 activity. Moreover, Pect-MCP significantly reduced integrin mRNA levels leading to reduced AKT activity.Serous Ovarian Cancer (SOC) Assignment