What is the difference between a genomic and a cDNA library?
What library would you want to screen if you needed to find a protein-encoding gene?
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2. What is the purpose of Sanger sequencing? What is the reason the ddNTPs used lack a 3’-hydroxyl group?
4. The questions below are based on RT-PCR:
a. How is mRNA purified from cells?
b. How does reverse transcriptase make cDNA? How is cDNA different that DNA? Difference between a genomic and a cDNA library.