Pharmacology Isolated Rat Ileum Tissue Essay
harmacology Laboratory Report on:
The muscular effect of acetylcholine, carbachol and butyrylcholine on isolated rat ileum tissue.
Module number: BMS242
Student Registration number: 170158804
Name: Adrianos Boutsias
Abstract word number: 247
Remainder report word number: 1,439. Pharmacology Isolated Rat Ileum Tissue Essay Paper
Neurotransmitters released by postganglionic neurones regulate smooth muscle activity in the gastrointestinal (GI) tract. An agonist is a substance, that, when bound to a receptor, initiates a physiological response, while an antagonist disallows a receptor’s wild-type function. The objectives of this study were to answer two questions: first, how does smooth muscle respond under influence from several neurotransmitters? and second, how quickly does the tissue contract under exposure to said neurotransmitters? In order to tackle these questions, acetylcholine, butyrylcholine and carbachol stocks were retrieved, diluted serially and pipetted into an organ bath, where their total bath concentrations were calculated: dose(g)molecular weight*1volume of bath. In the bath, isolated rat ileum, immersed in 20ml Krebs, was connected to a force transducer-micropositioner apparatus via a metal hook, allowing for a response (g) to be measured when varying concentrations of drugs were added.